No. 1688, Gaoke East Road, Pudong new district, Shanghai, China.
No. 1688, Gaoke East Road, Pudong new district, Shanghai, China.
Here we report on the development of an improved approach that combines neurogenin-2 programming with the use of commercially available midbrain differentiation kits for a rapid, efficient, and ...
Efficient derivation of large-scale motor neurons (MNs) from human pluripotent stem cells is central to the understanding of MN development, modelling of MN disorders in vitro and development of ...
Those three NTM cultures identified as MTBC by the Viasure kit were subjected to Sanger sequencing to confirm the MALDI-TOF MS result. Two strains were identified as NTM through the rpoB and 16S markers, while one of the strains could not be further characterized by Sanger sequencing.. Additionally, the specificity of the Viasure kit was 94.9% (CI 95%: …
However, LHCGR was not expressed, regardless of the treatment period. Therefore, the duration of the differentiation medium treatment for LLC differentiation was fixed to this intermediate period, i.e. on day 4 of treatment, but we speculated that additional factors might be needed to induce the expression of all markers.
In developing nervous tissue, the differentiation of astrocytes occurs later compared to neurons. It takes more time and more techniques to obtain mature and pure astrocytes in vitro. In this study, a protocol was developed to culture mature and pure astrocytes from human embryonic stem cells (hESCs). To obtain a high quantity and quality of ...
Astrocytes play a pivotal role in neuronal network development. Despite the well-known role of astrocytes in the pathophysiology of neurologic disorders, the utilization of human induced pluripotent stem cell (hiPSC)-derived astrocytes in neuronal networks remains limited. Here, we present a streamlined one-step protocol for the differentiation of hiPSCs directly into …
Here we report an orthogonally induced differentiation (OID) method for achieving genomically programmed human stem cells, organoids and 3D bioprinted organ-specific …
We describe a simple, rapid method to establish cultures from ARPE-19 cells, with significantly improved epithelial cell morphology and cytoskeletal organization, and RPE …
To achieve sustainable urban development, it is essential to gain insight into the spatial and temporal differentiation characteristics and the driving mechanisms of the urban thermal environment (UTE). As urbanization continues to accelerate, human activity and landscape configuration and composition interact to complicate the UTE. However, the …
With the bi-differentiation of the progenitors in the procallus, the angiogenesis caused by the initial hypoxia gradually relieves the hypoxic tension to promote further osteogenesis along with the MV priming condition, which mimics the whole stage from the initial inflammatory phase to subsequent reparative and remodeling phases illustrating ...
The results of PLS-DA model exhibited complete differentiation between Shahtoosh, Pashmina and Angora (Oryctolagus cuniculus domesticus) wool used for external validation study with highly significant predictive ability (R-square value 0.99). This proof-of-concept study illustrates the potential of ATR FT-IR spectroscopy to complement current ...
Differentiation protocols aim to recapitulate the development of lymphocytes. Hence, the induction of lymphopoiesis relies on stimulation by SCF, FLT3l, IL7, and Notch signaling (Moore and Zlotnik, 1997; Radtke et al., 1999, 2004; Politikos et al., 2015). Differentiation effectiveness can be assessed by surface markers.
In this paper, we describe the rapid differentiation of human pluripotent stem cells (both iPS and ES cells) using a synthetic mRNA cocktail …
Traditional methods of neural differentiation, such as induction and picking of neural rosettes and multi-step chronological addition of cytokines have resulted in the generation of various neural subtypes, including MSNs, but with high variability or excessive duration of differentiation [18–23].
Neurons derived from human induced Pluripotent Stem Cells (hiPSCs) provide a promising new tool for studying neurological disorders. In the past decade, many protocols for differentiating hiPSCs into neurons have been developed. However, these protocols are often slow with high variability, low repr …
Staphylococcus aureus encompasses 2 subspecies ( aureus and anaerobius) with significant differences in their epidemiology and pathogenicity. We evaluated the suitability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the rapid identification of both subspecies using a panel of 52 S. aureus isolates (30 subsp. anaerobius …
A critical need for the current PRV surveillance program in the United States is the rapid detection of PRV infection. For this reason, a set of 2 real-time polymerase chain reaction (PCR) assays by using TaqMan chemistry was developed and evaluated for their capability in the detection and differentiation of field and vaccine strains of PRV.
The differentiation of hPSCs takes place in two steps: first the induction of hematopoietic stem/progenitor cells (HSPCs), then the induction of lymphopoiesis by Notch signaling. However, the differentiation of T cells from hPSCs can be difficult and lack reproducibility. One parameter that needs to be better assessed is the potential of DLL1 ...
Human pluripotent stem cells (hPSCs) can generate a diversity of cell types, but few methods have been developed to derive cells of the kidney lineage. Here, we report a highly efficient system for differentiating human embryonic stem cells and induced pluripotent stem cells (referred to …
Early development is characterized by the rapid proliferation of embryonic cells, which then differentiate to produce the many specialized types of cells that make up the tissues and organs of multicellular animals.
Rapid and accurate identification of Salmonella enterica serotypes Typhi and Paratyphi (A, B and C), the causal agents of enteric fever, is critical for timely treatment, case management and evaluation of health policies in low and middle-income countries where the disease still remains a serious public health problem. The present study describes the …
Directed neuronal differentiation of human pluripotent stem cells (hPSCs), neural progenitors, or fibroblasts using transcription factors has allowed for the rapid and highly reproducible …
ARTICLE Rapid differentiation of hiPSCs into functional oligodendrocytes using an OLIG2 synthetic modified messenger RNA Jian Xu 1,4, Zhihua Yang 2,4, Rui Wang3,4, Fumei He1, Rong Yan3, Yidi ...
The period of NSC differentiation was significantly shortened under high EB density culture conditions. Simultaneous mass production of a pure population of NSCs was possible within 4 days. These ...
Sepsis is a life-threatening condition arising from a dysregulated host immune response to infection, leading to a substantial global health burden. The accurate identification of bacterial pathogens in sepsis is essential for guiding effective antimicrobial therapy and optimising patient outcomes. …
Sepsis is a life-threatening condition arising from a dysregulated host immune response to infection, leading to a substantial global health burden. The accurate identification of bacterial pathogens in sepsis is essential for …
We adapted protocols for differentiating human pluripotent stem cells to functional human cardiomyocytes in a 96-well microplate format. The resulting cardiomyocytes …
Overall, this integrated RT-ERA pre-amplification and Cas12a/crRNA cleavage assay is a practical tool for reliable and rapid detection of PEDV for diagnostic differentiation. Porcine epidemic diarrhea virus (PEDV) is an enteric coronavirus that causes acute watery diarrhea and vomiting in unweaned piglets, and is associated with
Research suggests that microbiomes play a major role in structuring plant communities and influencing ecosystem processes, however, the relative roles and strength of change of microbial ...
The protocol entails a total differentiation period of 4 days and comprises two steps: (i) differentiation of h-iPSCs into intermediate h-MPCs and (ii) conversion of h-MPCs into h-iECs upon delivery of modRNA encoding ETV2. We showed that this S1-modETV2 approach allows widespread expression of ETV2 throughout the entire h-MPC population, thus ...
